The primary structure of Pseudomonas cytochrome c peroxidase
- 3 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 250 (2) , 175-178
- https://doi.org/10.1016/0014-5793(89)80714-8
Abstract
The primary structure of Pseudomonas cytochrome c peroxidase is presented. The intact protein was fragmented with cyanogen bromide into five fragments; partial cleavage was observed at a Met-His bond of the protein. The primary structure was established partly by automatic Edman degradations, partly by manual sequencing of peptides obtained with trypsin, thermolysin, chymotrypsin, pepsin, subtilisin and Staphylococcus aureus V8 endopeptidase. The order of the cyanogen bromide fragments was further confirmed by overlapping peptides obtained by specific cleavage of the whole protein. Pseudomonas cytochrome c peroxidase consists of 302 amino acid residues giving a calculated M r of 33 690.Keywords
This publication has 18 references indexed in Scilit:
- Specific cleavage of Pseudomonas cytochrome-c peroxidase by elastase from Pseudomonas aeruginosaBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1987
- Amino acid sequences of the two heme c-binding sites of Pseudomonas cytochrome-c peroxidaseBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1987
- Structural basis for the variation of pH-dependent redox potentials of Pseudomonas cytochromes c-551Biochemistry, 1984
- Resonance Raman spectra of Pseudomonas cytochrome c peroxidaseBiochimica et Biophysica Acta (BBA) - Protein Structure, 1980
- Heme-linked properties of Pseudomonas cytochrome c peroxidase. Evidence for non-equivalence of the hemesBiochimica et Biophysica Acta (BBA) - Protein Structure, 1979
- High-yield cleavage of tryptophanyl peptide bonds by o-iodosobenzoic acidBiochemistry, 1979
- Covalent structure of the glycoprotein horseradish peroxidase (EC 1.11.1.7)FEBS Letters, 1976
- Pseudomonas Cytochrome c Peroxidase. IX. Molecular Weight of the Enzyme in Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis.Acta Chemica Scandinavica, 1973
- Structure of N-terminal Fragments of Fibrinogen and Specificity of ThrombinNature, 1967