The nucleotide sequence of the yeastMEL1gene
Open Access
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (20) , 7257-7268
- https://doi.org/10.1093/nar/13.20.7257
Abstract
The complete nucleotide sequence of the MEL1 gene of the yeast, Saccharomyces cerevisiae, encoding α-galactosidase was determined. The nucleotide sequence contains an open reading frame of 1413 bp encoding a protein of 471 amino acids. Comparison with the known N-terminal amino acid sequence of the mature secreted protein indicated that α-galactosidase is synthesized as a precursor with an N-terminal signal sequence of 18 amino acids. The general features of this signal peptide resemble those of other yeast signal peptides. Molecular weight of the mature α-galactosidase polypeptide deduced from the nucleotide sequence is 50.049 kd. The 5′ regulatory region has sequences in common with other yeast genes regulated by the GAL4-protein.Keywords
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