Abstract
Among hat-spored isolates of the genus Pichia collected in frass from trees in the U.S. and Japan were strains of two taxa that could not be identified with presently described species. These new homothallic species, described as P. hampshirensis (type strain, NRRL YB-4128 = CBS 7208) and P. japonica (type strain, NRRL YB-2750 = CBS 7209), show little nuclear DNA relatedness to P. wickerhamii, P. rhodanensis, P. mississippiensis, P. toletana, and other phenotypically similar taxa.