Synthesis and Electrophoretic Distribution of Antibodies in the Amphibian, Bufo Marinus.

Publisher Website
Google Scholar
Abstract
Adult specimens of the amphibian, Bufo marinus maintained at an ambient temperature of 25[degree] C were immunized with a mixture of Salmonella typhosa H. antigen and rabbit gamma globulin (RGG). Each animal produced precipitating antibody for RGG and agglutinating antibody for S. typhosa after a short period of immunization. When the antiserums were separated electrophoretically, the agglutinins were found in the slowest-moving of the 4 major components. On the basis of its electrophoretic mobility, this fraction would be considered analogous to the gamma globulin of mammals.