r Carotene Hydroxylase Gene from the Cyanobacterium Synechocystis sp. PCC6803
Open Access
- 1 May 1998
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 39 (5) , 560-564
- https://doi.org/10.1093/oxfordjournals.pcp.a029405
Abstract
The ORF sll1468 of Synechocystis sp. PCC6803 was identified as a gene for rβ-carotene hydroxylase by functional complementation in a rβ-carotene-producing Escherichia coll. The gene product of ORF sll11468 added hydroxyl groups to the rβ-ionone rings of rβ-carotene (rβ,rβ-carotene) to form zeaxanthin (rβ,rβ-carotene-3,3′-diol). This newly identified rβ-carotene hydroxylase does not show overall amino acid sequence similarity to the known rβ-carotene hydroxylases. However, it showed significant sequence similarity to rβ-carotene ketolases of marine bacteria and a green alga.Keywords
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