The effect of hydrocortisone on the incorporation of tritiated thymidine by human blood lymphocytes cultured with phytohaemagglutinin and pokeweed mitogen.

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  • 1 October 1973
    • journal article
    • Vol. 15  (2) , 203-12
Abstract
Methods based on [3H]thymidine incorporation and morphology were used for further studies on the effect of hydrocortisone on the transformation of human lymphocytes by phytohaemagglutinin (PHA) and pokeweed mitogen (PWM). Result obtained with both methods showed inhibition of PHA stimulation in cultures preincubated with 0·01–10 μg/ml of hydrocortisone. [3H]thymidine incorporation due to PWM in vertical tube cultures was depressed by 1·0 μg/ml of hydrocortisone in cultures harvested at 48 hr, but not in similar cultures incubated for 3, 4 and 5 days. In vertical cultures 10 μg/ml of hormone depressed the level of uptake in most experiments. By contrast [3H]thymidine incorporation was enhanced in horizontal PWM cultures by 0·1 and 1·0 μg/ml of hydrocortisone, and 10 μg/ml caused stimulation or a return to the normal PWM level.