Calcium and Calmodulin-Dependent Phosphorylation of κ-Casein by a Bovine Mammary Casein Kinase
Open Access
- 1 November 1987
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 70 (11) , 2226-2232
- https://doi.org/10.3168/jds.s0022-0302(87)80282-5
Abstract
A calcium and calmodulin-dependent .kappa.-casein kinase activity has been described in the bovine mammary gland. This kinase required previously dephosphorylated .kappa.-casein for substrate, thus suggesting a physiological role for this enzyme. The .kappa.-casein kinase required magnesium and the presence of both calcium and calmodulin for full activity. Calmodulin concentrations of .32 .mu.M achieved one-half maximal activation of this enzyme. The calcium and calmodulin-dependent .kappa.-casein kinase was found in preparations of mammary acini and could be localized in a membranous fraction by centrifugation. The particles containing this activity had a high density (1,309 g/cc) and cofractionated with caseins, suggesting this enzyme may be present in secretory granules.Keywords
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