Effect of O2tension on the bioactivation and metabolism of aliphatic halides by primary rat-hepatocyte cultures
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 14 (7) , 521-525
- https://doi.org/10.3109/00498258409151441
Abstract
1. The covalent binding of CCl4 and CF3CHBrCl to cultured rat hepatocytes was enhanced by anoxic conditions, indicative of enhanced reductive biotransformation. 2. The covalent binding of CHCl3 and CHCl2CH2Cl decreased as the O2 concentration was decreased, indicating a preference for oxidative metabolism. 3. Lower O2 tensions decreased the formation of polar water-soluble metabolites with CHCl3 and CHCl2CH2Cl, while CCl4 and CF3CHBrCl were unaffected. 4. The results indicate that primary cultured hepatocytes can reductively biotransform and bioactivate aliphatic halides under anoxic conditions.Keywords
This publication has 12 references indexed in Scilit:
- Correlations of in vitro and in vivo hepatotoxicity for five haloalkanesToxicology and Applied Pharmacology, 1983
- Chromatographic resolution of amino acid adducts of aliphatic halidesChemico-Biological Interactions, 1982
- Butylate hydroxytoluene pretreatment protects against cytotoxicity and reduces covalent binding of aflatoxin B1 in primary hepatocyte culturesToxicology and Applied Pharmacology, 1981
- Covalent binding of carbon tetrachloride metabolites to liver nuclear DNA, proteins, and lipids*1, *2Toxicology and Applied Pharmacology, 1980
- Deuterium isotope effect in in vivo bioactivation of chloroform to phosgeneBiochemical Pharmacology, 1979
- Maintenance of cytochrome P-450 and metabolism of aflatoxin B1 in primary hepatocyte culturesBiochemical and Biophysical Research Communications, 1977
- Bioactivation of carbon tetrachloride, chloroform and bromotrichloromethane: Role of cytochrome P-450Life Sciences, 1977
- N-demethylation as an example of drug metabolism in isolated rat hepatocytesBiochemical Pharmacology, 1976
- Isolated rat hepatocytes as an experimental tool in the study of cell injury. Effect of anoxiaForensic Science, 1976
- A comparative study on the irreversible binding of labeled halothane trichlorofluoromethane, chloroform, and carbon tetrachloride to hepatic protein and lipids in vitro and in vivoArchives of Toxicology, 1975