Untersuchungen zum Wirkungsmechanismus von Silibinin, V. Einfluß von Silibinin auf die Synthese ribosomaler RNA, mRNA und tRNA in Rattenlebern in vivo

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Abstract
The influence of the flavonolignane silibrinin on the rate of RNA synthesis in rat livers was studied in detail and the time course of the stimulatory effect was determined; 8 h after i.p. application a maximal increase of .apprx. 60% in nuclear RNA synthesis can be observed. The analysis of the RNA by electrophoresis on agarose and by sucrose gradient centrifugation demonstrated that the ribosomal RNA (28S, 18S, 5.8S) synthesis is accelerated followed by enhanced incorporation of rRNA into mature ribosomes. During stimulation changes in the pattern of 45 S RNA can be observed. The synthesis of mRNA, 5S RNA and tRNA was not influenced by silibinin, which was shown after separation of these moieties on olgio(dT [thymidine])-cellulose, and by polyacrylamide electrophoresis, respectively. The clinically observed enhancement of liver cell regeneration during silibinin treatment can apparently be explained by an increase of the protein synthetic apparatus.

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