Determination of Furazolidone in Chicken Tissues by High-Performance Liquid Chromatography

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Abstract
A method was established for the quantitative determination of furazolidone [an antimicrobial] agent in chicken tissues by high-performance liquid chromatography. An ethyl acetate extract of the sample was purified by alumina column chromatography with CCl4-isopropanol (1:1, vol/vol) as an eluate. The solvent was evaporated and the residue was dissolved in methanol-water (95:5, vol/vol) containing o-nitrophenol as an internal standard. An aliquot of the solution was injected into a Hitachi gel #3011 column. Furazolidone was separated from other substances by using methanol-water (95:5, vol/vol) as a mobile phase. Recovery of furazolidone from the muscle and liver added with it in levels of 0.01-0.1 ppm, was 68.9 and 68.5%, respectively.

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