A Urinary Pentasaccharide in Bovine Mannosidosis

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Abstract
Abnormally high amounts of low molecular weight mannose‐rich carbohydrate material were found in the urine of an Angus calf with mannosidosis. At least five oligosaccharide fractions were detected by paper chromatography. The most abundant compound was purified by gel chromatography, zone electrophoresis, and two consecutive preparative paper chromatographic steps. The yield was 10 mg/liter of urine. From structural studies including nuclear magnetic resonance spectroscopy, optical rotation, sugar analysis, methylation analysis, and partial enzymatic degradation the following structure was deduced: α‐d‐Manp‐(1→6)‐β‐d‐Manp‐(1→4)‐β‐d‐GlcNAcp‐(1→4)‐β‐d‐GlcNAcp‐(1→4)‐d‐GlcNAc. This oligosaccharide is distinct from all the oligosaccharides previously described which are excreted by patients with mannosidosis.