A radioimmunoassay for human parathyroid hormone utilizing a goat anti-bovine PTH serum

Publisher Website
Google Scholar
Abstract
An antiserum (NG-1) against bovine [b] PTH [parathyroid hormone] generated in the domestic goat was characterized for use in the radioimmunoassay of PTH in human [h] serum. When a carboxyterminal fragment of bPTH is used as radioligand, this antiserum detects only an antigenic site in the central region of the hPTH molecule. The synthetic hormone fragment, hPTH-(44-68), will displace 93% of the tracer, after which the addition of intact hPTH causes no further displacement. The assay does not detect the synthetic aminoterminal 1-34 fragment of the b or h hormones, nor the carboxyterminal fragment, hPTH-(53-84). Standard curves with bPTH-(1-84) and partially purified hPTH are not parallel, so that hPTH is used as standard. Serum from subjects with uremia or primary hyperparathyroidism gives dilution curves parallel to that with the hPTH standard. The assay with NG-1 was applied to the diagnosis of primary and secondary hyperparathyroidism, used to monitor the disappearance of PTH after parathyroidectomy and used to measure PTH in selective venous samples.

This publication has 0 references indexed in Scilit: