Identification of Neuron-Specific Enolase and Nonneuronal Enolase in Human and Rat Brain on Two-Dimensional Polyacrylamide Gels

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Abstract
The location of the enzymes neuron-specific enolase and nonneuronal enolase on 2-dimensional gels generated from tissue samples obtained from fresh human and rat cortex has been identified based on the following criteria: comigration on polyacrylamide gels with the appropriate purified protein and staining on nitrocellulose protein blots of human and rat cortex, using antibodies specific for each protein. This preparation of neuron-specific enolase from rat and human brain is highly pure, as only 1 spot is obtained on 2-dimensional gels. The antiserum to neuron-specific enolase is highly specific, as it reacts only with neuron-specific enolase on nitrocellulose blots derived from 2-dimensional gels of cortical tissue. The location of these proteins is of interest because it positively identifies 2 major brain proteins on 2-dimensional polyacrylamide gels of fresh cortical tissue. This information will be useful in a variety of future studies aimed at both identifying specific proteins on 2-dimensional gels and observing the effects of experimental manipulations on brain and other neuronal proteins.