ENHANCED EXPRESSION OF THE ESCHERICHIA-COLI SERA GENE IN A PLASMID VECTOR - PURIFICATION, CRYSTALLIZATION, AND PRELIMINARY-X-RAY DATA OF D-3 PHOSPHOGLYCERATE DEHYDROGENASE

  • 15 February 1989
    • journal article
    • research article
    • Vol. 264  (5) , 2645-2648
Abstract
The serA gene of Escherichia coli strain K-12, which codes for the cooperative allosteric enzyme D-3-phosphoglycerate dehydrogenase, was inserted into an inducible expression vector which produced phosphoglycerate dehydrogenase as 8% of the soluble protein of E. coli. The purified protein was used to grow several different single crystal forms. One of these, with space group P21, appears to contain all four subunits of the tetrameric enzyme in the asymmetric unit and diffracts to sufficient resolution to allow determination of the structure of phosphoglycerate dehydrogenase.