Amygdalar and hippocampal substrates of anxious temperament differ in their heritability

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Abstract
An anxious temperament in both humans and monkeys is evident from infanthood, is an important risk factor for later psychopathology, and is known to be heritable. In a large-scale study combining imaging with genetics, Oler et al. characterize the neural circuitry associated with this trait and the extent to which the function of this circuit is heritable. They scanned more than 200 related monkeys from a single-family pedigree using positron emission tomography after exposing the monkeys to a mildly stressful situation. Activation in both the amygdala and hippocampus was predictive of anxious temperament, but heritability of hippocampal activity was greater than that for amygdala. This suggests that there may be different effects of genes and environment on the function of these two regions in anxious temperament, and provides new insights into the genetic risk for anxiety and depressive disorders. Anxious temperament in both humans and monkeys is an important early predictor of psychopathology and is known to be heritable. These authors characterize the neural circuitry associated with this trait and the extent to which its function is heritable. A scan of related monkeys after exposure to mild stress showed that activation in both the amygdala and hippocampus was predictive of anxious temperament, but that heritability of activity in hippocampus was greater than that in amygdala. Anxious temperament (AT) in human and non-human primates is a trait-like phenotype evident early in life that is characterized by increased behavioural and physiological reactivity to mildly threatening stimuli1,2,3,4. Studies in children demonstrate that AT is an important risk factor for the later development of anxiety disorders, depression and comorbid substance abuse5. Despite its importance as an early predictor of psychopathology, little is known about the factors that predispose vulnerable children to develop AT and the brain systems that underlie its expression. To characterize the neural circuitry associated with AT and the extent to which the function of this circuit is heritable, we studied a large sample of rhesus monkeys phenotyped for AT. Using 238 young monkeys from a multigenerational single-family pedigree, we simultaneously assessed brain metabolic activity and AT while monkeys were exposed to the relevant ethological condition that elicits the phenotype. High-resolution 18F-labelled deoxyglucose positron-emission tomography (FDG–PET) was selected as the imaging modality because it provides semi-quantitative indices of absolute glucose metabolic rate, allows for simultaneous measurement of behaviour and brain activity, and has a time course suited for assessing temperament-associated sustained brain responses. Here we demonstrate that the central nucleus region of the amygdala and the anterior hippocampus are key components of the neural circuit predictive of AT. We also show significant heritability of the AT phenotype by using quantitative genetic analysis. Additionally, using voxelwise analyses, we reveal significant heritability of metabolic activity in AT-associated hippocampal regions. However, activity in the amygdala region predictive of AT is not significantly heritable. Furthermore, the heritabilities of the hippocampal and amygdala regions significantly differ from each other. Even though these structures are closely linked, the results suggest differential influences of genes and environment on how these brain regions mediate AT and the ongoing risk of developing anxiety and depression.