High Performance Liquid Chromatography Determination of Cystathionase Activity in Human and Rabbit Lenses
- 1 January 1986
- journal article
- research article
- Published by S. Karger AG in Ophthalmic Research
- Vol. 18 (6) , 343-348
- https://doi.org/10.1159/000265461
Abstract
Cystathionase activity has been demonstrated in human and rabbit lenses, using L-homoserine as substrate. The amount of activity found was quite low, 0.05 and 0.08 nmol of α-ketobutyrate formed/h/mg protein in human and rabbit lenses, respectively. The human lens cystathionase was found to have a pH maximum of 8.5 and a Km of 23.8 mM for L-homoserine. A sensitive assay, involving the use of high performance liquid chromatography, was developed to measure the small amounts of α-ketobutyrate produced.Keywords
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