Evaluation of Test Sensitivity and Test Specificity of Three Field Methods for Quantification of Chloroquine in Urine Using Liquid Chromatography as Reference Method

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Abstract
We have evaluated three quantitative colorimetric methods [bromthymol blue (BTB), Haskins, and Saker-Salomons (S-S)] for measurement of concentrations of chloroquine (CQ) in urine. A graphical evaluation of test sensitivity and test specificity at different concentrations of CQ in authentic urine samples was used. Liquid chromatography (LC) was used as a reference method. The Haskins method showed the highest test specificity (89%) and test sensitivity (97%) as the Haskins method, S-S and BTB methods had test specificities of 49 and 13%, respectively. The correlation coefficients between the LC method and the colorimetric methods were all higher than 0.94 at a urinary concentration range of 2-100 .mu.ol/L. The intra- and interassay variations for the colorimetric methods were all uniformly <15% at 2 .mu.mol/L, but the Haskins method showed less variation than the other two methods.

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