STABLE ISOLATED SYMMETRICAL ANHYDRIDES OF Nα‐9‐FLUORENYLMETHYLOXYCARBONYLAMINO ACIDS IN SOLID‐PHASE PEPTIDE SYNTHESIS:
- 1 September 1981
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 18 (3) , 237-241
- https://doi.org/10.1111/j.1399-3011.1981.tb02977.x
Abstract
The synthesis and isolation of symmetrical anhydrides of Nα‐9‐fluorenylmethyloxycarbonyl (Fmoc) amino acids using water soluble carbodiimide is described. These compounds were used in a solid phase peptide synthesis of methionine‐enkephalin on a p‐benzyloxybenzyl ester polystyrene 1% divinylbenzene resin support. Homogeneous free pentapeptide was obtained in 42% overall yield. The Fmoc amino acid symmetrical anhydrides were stable during prolonged storage (2 years at 0°) and offer advantages over present “Fmoc solid phase” methods which use anhydrides formed in situ.Keywords
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