Application of the Syva EMIT and Abbott TDx Amphetamine Immunoassays to the Detection of 3,4-Methylenedioxymethamphetamine (MDMA) and 3,4-Methylenedioxyethamphetamine (MDEA) in Urine

Publisher Website
Google Scholar
Abstract
MDMA and MDEA are hallucinogenic analogs of amphetamine. The need for laboratory monitoring of these substances has developed as a result of their increased recreational use. Since the Abbott TDx® and Syva EMIT®-d.a.u.™ immunoassays are commonly used tests for urine monitoring of drugs-of-abuse, the amphetamine assay of each manufacturer was assessed to determine the degree of cross-reactivity with MDMA and MDEA. Cross-reactivity was evaluated using a series of MDMA- and MDEA-spiked urine samples. Testing was performed over a two-day period with 3 runs/day and each sample run in duplicate. The Syva EMIT d.a.u. amphetamine assay was positive only at the highest concentration standard (10.0 µg/mL) for both MDMA and MDEA. Consequently, no further testing was performed with this assay. Calibration curves were generated for the TDx runs and percent cross-reactivity determinations were made. Precision for the TDx data was evaluated based on within-day and between-day coefficients of variation (CV). CVs for MDMA runs were below 6% for within-day and below 5% for between-day runs. Values of CV for MDEA were below 16% for both within-day and between-day runs; CVs were less than 2.5% for positive values. Cross-reactivity for MDMA ranged from 18% (10.00 µg/mL) to 118% (0.15 vg/mL). Cross-reactivity for the MDEA standards ranged from 12% (10.00 µg/mL) to 47% (0.15 µg/mL). The presence of MDMA and/or MDEA in samples resulting in a negative EMIT-d.a.u. test and a positive TDx test was confirmed by GC/MS analysis. The urine sample was extracted and then derivatized with heptafluorobutyric anhydride prior to analysis with a Hewlett-Packard Model 5988A gas chromatograph/mass spectrometer using the electron impact-selected ion monitoring mode.