Proliferation Kinetics of A Human Malignant Melanoma Serially Grown In Nude Mice

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Abstract
The technique of labeled mitoses and flow cytometric DNA analysis were used to determine the proliferation kinetics of a human malignant melanoma grown in nude mice. The effect of tumor volume and of long-term serial transplantation on the kinetic parameters was investigated. The cell loss factor, which was the dominant factor in the growth of this melanoma, increased from 52-69% with increasing tumor size, whereas the calculated growth fraction showed no systematic changes. The cell generation time increased from 34 to 44 h with tumor size, mainly due to a prolongation of the G1 duration time, whereas no significant changes occurred in the duration of the S and G2 phases of the cell cycle. The stability of the investigated tumor characteristics indicated that the kinetics of this melanoma remained unchanged during more than 60 serial transplantations in nude mice. The methods applied are suitable for a detailed description of tumor growth kinetics, since they provide complementary results.