Immunohistochemical Localization of 2′,3′‐Cyclic Nucleotide 3′‐Phosphodiesterase and Myelin Basic Protein in the Central Nervous System of the Jimpy and the Normal Mouse
- 1 March 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (3) , 686-691
- https://doi.org/10.1111/j.1471-4159.1985.tb12869.x
Abstract
We describe the immunohistochemical localization of 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase (CNPase) and myelin basic protein (MBP) in CNS of the jimpy mutant mouse which is characterized by dys‐ and demyelination. In controls, the CNPase and MBP were localized exclusively in white matter in the CNS. The jimpy mutant mice were severely affected: A very weak reaction was observed in the white matter. Very few CNPase‐ and MBP‐positive myelin sheaths were observed, and some degradation products were also observed after reaction with antisera against both CNPase and MBP. The immunohistochemical reaction in the jimpy mice showed a similar localization in both CNPase and MBP.Keywords
This publication has 25 references indexed in Scilit:
- Immunochemical Identification of 2′, 3′‐Cyclic Nucleotide 3′‐Phosphodiesterase in Central and Peripheral Nervous System Myelin, the Wolfgram Protein Fraction, and Bovine OligodendrocytesJournal of Neurochemistry, 1983
- Immunohistochemical Localization of 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase and Myelin Basic Protein in the Chick RetinaJournal of Neurochemistry, 1983
- Intracellular Localization of 2′,3′‐Cyclic Nucleotide 3′‐Phosphodiesterase in a Neuronal Cell Line as Examined by Immunofluorescence and Cell FractionationJournal of Neurochemistry, 1981
- Characterization of 2′:3′‐Cyclic Nucleotide 3′‐Phosphodiesterase: Limited Proteolytic Digestion, Plant Lectin Affinity Chromatography, and Immunological IdentificationJournal of Neurochemistry, 1981
- Subcellular Distribution and Developmental Change of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in the Central Nervous System of the Myelin‐Deficient Shiverer Mutant MiceJournal of Neurochemistry, 1980
- A Rapid Micro Method for 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase Assay Using Micro High Performance Liquid ChromatographyJournal of Neurochemistry, 1980
- Improved Method for Prufication of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase from Bovine Cerebral White MatterJournal of Neurochemistry, 1980
- PURIFICATION OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE FROM BOVINE BRAIN BY IMMUNOAFFINITY CHROMATOGRAPHY: FURTHER BIOCHEMICAL CHARACTERIZATION OF THE PROTEINJournal of Neurochemistry, 1979
- PURIFICATION AND COMPARISON OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASES FROM BOVINE BRAIN AND SPINAL CORDJournal of Neurochemistry, 1978
- Carbodiimides. VIII.1 Observations on the Reactions of Carbodiimides with Acids and Some New Applications in the Synthesis of Phosphoric Acid EstersJournal of the American Chemical Society, 1958