Ultrastructural aspects of coated vesicles in tobacco protoplasts

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Abstract
The ultrastructure and distribution of coated vesicles in isolated tobacco protoplasts were investigated using transmission electron microscopy of thin sections of whole protoplasts and stained plasma membrane preparations obtained by osmotic bursting of protoplasts attached to coated microscope grids. Large numbers of coated vesicles were associated with both the plasma membrane and the maturing face of dictyosomes. Dictyosome associated coated vesicles were smaller and had less distinct coats and vesicle membranes than those associated with the plasma membrane. Honeycomb structures believed to be aggregations of coats were also associated with the inner surface of the plasma membrane. Our data suggest that coated vesicles are produced by the Golgi apparatus, fuse with the plasma membrane, their coats remaining attached, at least temporarily, to the plasma membrane inner surface.

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