INVITRO SYNTHESIS OF IGE BY HUMAN PERIPHERAL-BLOOD LEUKOCYTES - RELATIONSHIP TO SERUM TITER OF LEUKOCYTE DONOR

  • 1 January 1981
    • journal article
    • research article
    • Vol. 43  (3) , 458-462
Abstract
A microculture system was developed for in vitro IgE synthesis employing human peripheral blood leucocytes (PBL), comprising 106 cells in 1.0 ml serum-supplemented growth medium, in round-bottomed tubes. Examination of the kinetics IgE synthesis indicated that production was relatively linear during culture for up to 12 days, and was not susceptible to stimulation with polyclonal B cell mitogen. Employing a standardized 7 day culture period, PBl IgE synthesis was compared within a group of 50 volunteers, comprising normal non-atopic individuals exhibiting serum IgE titers < 50 U/ml, and moderate atopics with serum titers up to approximately 400 U/ml. PBL IgE synthesis was found to reflect closely the cell donor serum IgE status, a feature thus far unique to this culture system, and indicative of the preservation of normal in vivo regulatory mechanisms in the in vitro cultures.