Genetic analysis of anther-derived plants of rice: independent assortment of unlinked genes

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Abstract
Haploid and diploid plants produced from anther culture of two hybrids of rice (Oryza sativa), each heterozygous for two unlinked genes, were subjected to genetic analysis. Progeny tests showed that all diploids but one were homozygous for both loci. Chi-square tests of the haploids and homozygous diploids revealed that the two genes in each hybrid segregated in the expected 1:1 ratio and that the four genotypic classes fit a 1:1:1:1 ratio, indicating that independent assortment of marker genes occurred and that the gametes were equally viable in the microspore-derived progeny. One diploid plant produced from anthers of the hybrid of genotype wx+wx lg+lg was found to be homozygous for lg+ but heterozygous for the waxy locus. Indirect evidence suggests that a mutation at the waxy locus in a spontaneously doubled haploid cell during culture may be the cause for occurrence of this unusual plant.

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