Effect of elongation factor Tu on the conformation of phenylalanyl‐tRNAPhe
- 5 April 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 154 (1) , 42-46
- https://doi.org/10.1016/0014-5793(83)80871-0
Abstract
Structural features of the tRNAPhe molecule upon ternary complex formation with the bacterial elongation factor Tu were investigates. Phosphodiester bonds at positions 18 and 34 were found to be labilized in bound tRNA. Conversely, a higher stability of the phosphodiester links at positions 20, 21 and 36 was detected. Using ethylnitrosourea as a chemical probe a conformational change occurring at phosphate position 53 was observed in complexed tRNA. These results are interpreted by a structural rearrangement of the nucleic acid induced by complex formationKeywords
This publication has 23 references indexed in Scilit:
- Chemical probes for tRNA tertiary structureFEBS Letters, 1982
- A study of the interaction of Escherichia coli elongation factor-Tu with aminoacyl-tRNAs by partial digestion with cobra venom ribonucleaseJournal of Molecular Biology, 1981
- Tertiary Structure of tRNAs in Solution Monitored by Phosphodiester Modification with EthylnitrosoureaEuropean Journal of Biochemistry, 1981
- Fluorimetric Study of the Complex between Yeast Phenylalanyl‐tRNA Synthetase and tRNAPheEuropean Journal of Biochemistry, 1980
- The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methodsFEBS Letters, 1978
- Isolation, characterization and structural implications of a nuclease-digested complex of aminoacyl transfer RNA and Escherichia coli elongation factor TuJournal of Molecular Biology, 1977
- Complete purification and studies on the structural and kinetic properties of two forms of yeast valyl-tRNA synthetaseBiochimie, 1976
- Modification of Phenylalanyl-tRNA Synthetase from Baker's Yeast by Proteolytic Cleavage and Properties of the Trypsin-Modified EnzymeEuropean Journal of Biochemistry, 1975
- Studies on tRNA Nucleotidyltransferase from Baker's YeastEuropean Journal of Biochemistry, 1974
- Effect of the presence of a pCpCpCA 3′‐terminus in Phe‐tRNAPheyeast on the interaction with elongation factors and with the poly U‐ribosome systemFEBS Letters, 1972