UPTAKE OF INTRAVENOUSLY ADMINISTERED PROGESTERONE, PREGNANEDIONE AND PREGNANOLONE BY THE RAT BRAIN
- 1 March 1968
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 57 (3) , 395-404
- https://doi.org/10.1530/acta.0.0570395
Abstract
A method has been developed for the detection, isolation and quantitation of progesterone, pregnanolone and pregnanedione in brain tissue of rats receiving pharmacological hypnotic doses of these 3 substances. Prior to extraction radioactive tracers were added to brain tissue obtained from animals receiving these agents and the brain tissue extracts were purified by paper and thin layer chromatography. The material administered as well as some of the major metabolites were quantitated by gas liquid chromatography [GLC], and corrections for losses throughout the entire procedure and up to injection into GLC were based on recovery of added radioactive markers. The possible significance of the conversion of progesterone into hypnotically more potent metabolites is discussed.This publication has 3 references indexed in Scilit:
- THE METABOLIC FATE OF C14-PROGESTERONE IN HUMAN SUBJECTS*Journal of Clinical Endocrinology & Metabolism, 1958
- PROGESTERONE “ANESTHESIA” IN HUMAN SUBJECTSJournal of Clinical Endocrinology & Metabolism, 1954
- CORRELATIONS BETWEEN THE CHEMICAL STRUCTURE AND THE PHARMACOLOGICAL ACTIONS OF THE STEROIDS1Endocrinology, 1942