Quantitative Study on Foci of Altered Liver Cells Induced in the Rat by a Single Dose of Diethylnitrosamine and Partial Hepatectomy2

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Abstract
After a single dose of 10 mg diethylnitrosamine/kg rat, foci of liver cells changed in adenosine triphosphatase, glucose-6-phosphatase, cytoplasmic basophilia, and glycogen storage. A method for the quantitation of the number and size distribution of these foci was derived. The number of adenosine triphosphatase-deficient lesions was increased five-fold to tenfold by partial hepatectomy 20–24 hours before application of the carcinogen. The number of lesions remained constant from 6 weeks to 8 months after induction, but the continuous increase in size suggested a certain autonomy of growth. Two phases of different growth rate could be distinguished. At the end of the 8-month experiment, about 4% of the liver tissue consisted of altered liver cells. The results may be related to the general problem of hepatocarcinogenesis, since the observed changes are also found in liver treated with other hepctoccrcincgens, and the changed cells could be tumor-cell precursors.

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