The preparation of polyamide-oligonucleotide probes containing multiple non-radioactive labels
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (3) , 501-505
- https://doi.org/10.1093/nar/18.3.501
Abstract
Oligonucleotide probes containing multiple nonradioactive labels have been prepared by utilising and extending the methods used to prepare polyamide-oligonucleotide conjugates. The probes were prepared by incorportaing suitable amino acid residues, such as lysines, in the polyamide, which were then used as sites for the attachment of the non-radioactive labels. The procedures developed give control over the distance of the label from the oligonucleotide, and also the inter-label distance. The labels can be conveniently introduced while the substrate is still on the solid support. Even though fluorescent oligonucleotide probes prepared in this way carrying multiple carboxyfluorescein labels gave low levels of fluorescence due to quenching, the probes containing ten biotin labels gave a detection sensitivity of approximately 5 attomole (3 million molecules).This publication has 6 references indexed in Scilit:
- Kallikreins, kinins and growth factor biosynthesisTrends in Biochemical Sciences, 1988
- Studies of Synthetic Polymers by Nonradiative Energy TransferScience, 1988
- A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probesNucleic Acids Research, 1988
- Efficient methods for attaching non-radioactive labels to the 5′ ends of synthetic oligodeoxyribonucleotidesNucleic Acids Research, 1986
- Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use hybridization probesNucleic Acids Research, 1986
- Irreversible Inhibition of Biotin Transport in Yeast by Biotinyl- p -nitrophenyl EsterProceedings of the National Academy of Sciences, 1971