Pollak's Trichrome Stain for Demonstrating Distemper Inclusion Bodies in Tissue Sections

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Abstract
Pollak''s trichrome stain has been found to give good polychromatic differentiation between the inclusion body and the cellular elements. This method has been used with excellent results in staining sections taken from cases of mink, fox, and ferret distemper. After the sections have been prepared and passed through the alcohols in the usual manner, they are stained in Weigert''s iron-hematoxylin for 4-8 min., after which they are washed in running water for the same length of time. They are then stained in Pollak''s trichrome for 7-15 min., the intensity of the stain being checked by the use of the microscope. Following rapid differentiation in 0.2% acetic acid and rapid dehydration in 95% and absolute alcohol, the sections are cleared in xylene and mounted. If staining is satisfactory, inclusion bodies should stain crimson. Nuclei stain purple, and the cytoplasm of the bladder and trachea epithelial cells grayish-purple. Red blood cells stain orange; collagen stains blue-green and muscle red.

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