Cholesterol Estimation on Unmeasured Drops of Whole Blood.

Abstract
A method is presented for estimation of serum total cholesterol concentration from concentration of cholesterol/unit of whole blood solids, using a few unmeasured drops of finger tip blood dried in room air on filter paper. Prolonged storage at ordinary temperatures may elapse before analysis. In 124 sets of comparisons between serum from arm vein and dried finger tip blood, standard error of measurement was [plus or minus] 15.5 mg cholesterol/100 ml serum. This is comparable to intra-individual variability in direct serum measurement of blood samples drawn a few days apart and is smaller than variability between serum values in casual bloods drawn at longer intervals. Significance of blood cholesterol level in prediction of risk from coronary heart disease has been under-estimated in previous studies.

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