Membrane association of the carbon monoxide oxidation system in Rhodopseudomonas gelatinosa
- 1 January 1983
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 153 (1) , 571-3
- https://doi.org/10.1128/jb.153.1.571-573.1983
Abstract
A comparison of the distribution of CO oxidation activity between soluble and particulate protein fractions obtained after disruption of CO-grown Rhodopseudomonas gelatinosa 1 by French pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell membrane. An improved, strictly anaerobic method is given for spectrophotometric measurement of CO oxidation activity based on the carbon monoxide:methyl viologen oxidoreductase reaction.Keywords
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