ATP breakdown and muscle contraction

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Abstract
I Should like to consider first the possibility of showing, by chemical methods, that dephosphorylation of ATP is associated with normal contraction in a single twitch or a short series of twitches. There is some hope that this could be done, using the enzymatic spectrophotometric methods, worked out by Kalckar (1947) for purine analysis: the experiments would, of course, have to be done at 0°C, and on slow moving muscle as Professor Hill has suggested. In visualizing the following method I am greatly indebted to Professor Kalckar for his advice, The intense absorption in the ultra-violet, which is characteristic of the purines, occurs always in the region between 250 and 290 m µ, but the absorption maxima vary in height and in position for the different compounds. The method suggested for attacking this problem is to follow the production of ADP. ADP has the same absorption spectrum as ATP; but if it is acted upon by purified myokinase and purified adenylic deaminase, it is converted to the extent of 50% into inosinic acid:

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