The mutagenic activity of 5‐bromo‐2′‐deoxyuridine (BrdU) in vivo in rats
- 1 January 1983
- journal article
- research article
- Published by Wiley in Environmental Mutagenesis
- Vol. 5 (5) , 695-703
- https://doi.org/10.1002/em.2860050508
Abstract
BrdU tablets were implanted subcutaneously in rats, and BrdU concentrations were determined in the serum. Within 5 hr peak concentrations of 10 μg BrdU/ml blood were reached. The influence of BrdU in vivo on cell cycling, DNA synthesis, spontaneous sister chromatid exchange (SCE) frequencies, and gene‐mutation frequencies (6‐TGr) was determined in freshly isolated cells from a subcutaneous granulation tissue. The most significant effect of BrdU in vivo was a doubling of the spontaneous 6‐TGr frequency.In reconstruction experiments in vitro the mutagenic activity of BrdU applied in concentrations found in vivo was 2.5–6‐fold higher.With the use of agar‐coated tablets, BrdU concentrations in the blood were reduced by half, and no peak concentration was found. The differential staining of chromatids was still sufficient. Since the mutagenic effect of BrdU in vitro was found to be strongly concentration dependent, the use of agar‐coated tablets is recommended in experiments in which the compound is used to demonstrate SCE in vivo.Keywords
This publication has 17 references indexed in Scilit:
- Tables for determining the statistical significance of mutation frequenciesPublished by Elsevier ,2003
- Genetic effects of deoxyribonucleotide pool imbalancesEnvironmental Mutagenesis, 1982
- Sister chromatid exchange in vivo in mice: I. The influence of increasing doses of bromodeoxyuridineEnvironmental Mutagenesis, 1980
- Spontaneous and mitomycin-C induced sister-chromatid exchangesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Cell-cycle dependency of BudR-induced mutation for six genetic markers in cultured mammalian cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Demonstration of spontaneous sister chromatid exchanges in vivoExperimental Cell Research, 1976
- Stimulation of a subpopulation of hamster lymphoid cells by trypsin and chymotrypsinExperimental Cell Research, 1976
- Mutagenic mechanism of 5-bromodeoxyuridine in Chinese hamster cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1976
- New Giemsa method for the differential staining of sister chromatidsNature, 1974
- Release from Density Dependent Growth Inhibition by Proteolytic EnzymesNature, 1970