Regulation of LacZ mRNA translatability in a cell‐free system at heat shock by the last four sense codons

Abstract
Under heat shock conditions translation of Xenopus laevis normal mRNAs in a rabbit reticulocyte cell‐free system is blocked whereas hsp70 mRNA is translated. mRNA for E. coli β‐galactosidase containing the last four sense codons of Drosophila hsp70 at its 3′‐end was constructed. This mRNA is efficiently translated in a rabbit reticulocyte cell‐free system at 43°C.