Calcium‐activated, phospholipid‐dependent protein kinase in cultured rat mesangial cells
Open Access
- 11 November 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 192 (1) , 155-158
- https://doi.org/10.1016/0014-5793(85)80063-6
Abstract
The analysis of the 100000 × g supernatant fraction of cultured rat glomerular mesangial cells with DEAE‐cellulose ion‐exchange chromatography revealed a large peak showing the activity of a protein kinase (protein kinase C) which depended on phospholipid and diolein as well as Ca2+ Furthermore, it was shown that angiotensin II (AII) (10−6 M) induced rapid hydrolysis of phosphatidylinositol 4,5‐bisphosphate, leading to production of diacylglycerol rich in arachidonic acid, in the cultured rat mesangial cells. These results suggest that activation of protein kinase C resulting from enhancement of phosphoinositide metabolism may be important as an intracellular regulatory mechanism of AII upon cultured mesangial cells.Keywords
This publication has 20 references indexed in Scilit:
- Prostaglandin synthesis by rat glomerular mesangial cells in culture. Effects of angiotensin II and arginine vasopressin.Journal of Clinical Investigation, 1983
- Chemotactic factor causes rapid decreases in phosphatidylinositol,4,5-bisphosphate and phosphatidylinositol 4-monophosphate in rabbit neutrophilsBiochemical and Biophysical Research Communications, 1983
- Glomerular cells in cultureKidney International, 1983
- Phosphatidylinositol turnover in platelet activation; Calcium mobilization and protein phosphorylationCell Calcium, 1982
- Ca2+-phospholipid dependent phosphorylation of smooth muscle myosinBiochemical and Biophysical Research Communications, 1982
- Inhibitory action of guanosine 3′,5′-monophosphate on thrombin-induced phosphatidylinositol turnover and protein phosphorylation in human plateletsBiochemical and Biophysical Research Communications, 1981
- Evidence for mesangial glomerular receptors for angiotensin II linked to mesangial cell contractilityFEBS Letters, 1980
- Phospholipid turnover as a possible transmembrane signal for protein phosphorylation during human platelet activation by thrombinBiochemical and Biophysical Research Communications, 1980
- Contraction of Cultured Rat Glomerular Cells of Apparent Mesangial Origin after Stimulation with Angiotensin II and Arginine VasopressinJournal of Clinical Investigation, 1980
- Isolation and characterization of rat glomerular epithelial cells in vitroKidney International, 1978