Arthrobacter Endo‐β‐N‐Acetylglucosaminidase Shows Transglycosylation Activity on Complex‐Type N‐Glycan Oxazolines: One‐Pot Conversion of Ribonuclease B to Sialylated Ribonuclease C
- 30 June 2010
- journal article
- research article
- Published by Wiley in ChemBioChem
- Vol. 11 (10) , 1350-1355
- https://doi.org/10.1002/cbic.201000242
Abstract
Transforming protein glycosylation: The Arthrobacter endo‐β‐N‐acetylglucosaminidase (Endo‐A) was found to transglycosylate complex N‐glycan oxazolines without product hydrolysis. Given its high activity in hydrolyzing high‐mannose‐type N‐glycans, the newly discovered property enables a one‐pot switch of glycans in a glycoprotein, as shown by the conversion of heterogeneous RNase B to homogeneous sialylated RNase C.Keywords
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