A New Method for the Determination of γ-Glutamyltransferase in Serum

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Abstract
A new, simple and sensitive method is described for assay of serum .gamma.-glutamyltransferase activity based on the hydrolysis of the substrate L-.gamma.-glutamyl-3-carboxy-4-nitroanilide which offers the advantage of producing directly its own chromogen. The substrate is highly soluble and the method can therefore easily be adapted to any equipment for the automated assay of .gamma.-glutamyltransferase. The activities at measured optimum substrate concentration are slightly higher than with the Szasz (1969) method in which L-glutamyl-p-nitroanilide is used as substrate.