A problem in the radiochemical assay of glucose-6-phosphatase in muscle
- 1 March 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 218 (2) , 649-651
- https://doi.org/10.1042/bj2180649
Abstract
A radiochemical assay for glucose-6-phosphatase, which depends on the absorption of radiolabelled substrate on to a nascent precipitate of BaSO4 and Zn(OH)2, can give rise to erroneously high activities. Adsorption on to a Fe(OH)3 precipitate does not expose the incubation medium to extreme alkaline conditions and provides a satisfactory and simple assay. This method demonstrates a very low activity of the enzyme in mammalian muscle, in contrast with previous findings.This publication has 7 references indexed in Scilit:
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