Survival of Avian Influenza Virus H7N2 in SPF Chickens and Their Environments
- 1 September 2003
- journal article
- Published by American Association of Avian Pathologists (AAAP) in Avian Diseases
- Vol. 47 (s3) , 1015-1021
- https://doi.org/10.1637/0005-2086-47.s3.1015
Abstract
The survival or clearance of the avian influenza virus (AIV) of subtype H7N2 in its chicken host was evaluated using experimentally infected specific pathogen free (SPF) chickens of different age groups. Birds of different ages were successfully infected with infectious doses ranging between 104.7 and 105.7 ELD50 per bird. In infected birds, the infective virus was undetectable usually by the third week following exposure. The infectivity or inactivation time of the H7N2 AIV in various environmental conditions was studied using chicken manure, heat, ethanol, pH, and disinfectants. The H7N2 AIV was effectively inactivated by field chicken manure in less than a week at an ambient temperature of 15–20°C. At a pH 2, heating at 56°C, and exposure to 70% ethanol or a specific disinfectant, the AIV infectivity was destroyed in less than 30 min. Resumen Sobrevivencia del virus de influenza aviar del tipo H7N2 en aves libres de patógenos específicos y el medio ambiente donde se crían las mismas. Se evaluó la sobrevivencia o eliminación del virus de la influenza aviar del tipo H7N2 en aves de varias edades libres de patógenos específicos, las cuales fueron infectadas en forma experimental. Las aves de diferentes edades fueron infectadas exitosamente mediante la aplicación de inóculos que contenían entre 104.7 y 105.7 dosis letales embrionarias 50 por ave. El virus no pudo ser detectado en las gallinas infectadas después de la tercera semana después de la inoculación. Se evaluó la infectividad o inactivación del virus de influenza sometido a varias condiciones del medio ambiente mediante el uso de heces, calor, etanol, pH y desinfectantes. E l virus de influenza del tipo H7N2 fue inactivado efectivamente por las heces de las gallinas en menos de una semana a temperaturas ambientes entre los 15 a 20 C. A un pH 2.0, el calentamiento a 56 C y la exposi...Keywords
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