Functional expression of the mutants of the chloroplast tRNALys gene from the liverwort, Marchantia polymorpha, in Escherichia coli
- 4 June 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 265 (1-2) , 59-62
- https://doi.org/10.1016/0014-5793(90)80883-k
Abstract
The anticodon of the tRNALys gene (trnK) in the liverwort, Marchantia polymorpha, was anificially converted to an amber anticodon. This mutant tRNALys(CTA) gene carrying either the intron or the C27‐C43 mismatch at the anticodon‐stem is not functional in Escherichia coli, but without both of them, it does work as a tRNALys amber suppressor.Keywords
This publication has 13 references indexed in Scilit:
- [19] Rapid and efficient site-specific mutagenesis without phenotypic selectionPublished by Elsevier ,2004
- Compilation of tRNA sequences and sequences of tRNA genesNucleic Acids Research, 1989
- Structure and organization of Marchantia polymorpha chloroplast genomeJournal of Molecular Biology, 1988
- Structure and organization of Marchantia polymorpha chloroplast genomeJournal of Molecular Biology, 1988
- Mischarging mutants of Su+2 glutamine tRNA in E. coli. I. Mutations near the anticodon cause mischarging.The Japanese Journal of Genetics, 1988
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Identification of transfer RNA suppressors in Escherichia coliJournal of Molecular Biology, 1984
- Extensive sequence homology in the DNA coding for elongation factor Tu from Escherichia coli and the Chlamydomonas reinhardtii chloroplast.Proceedings of the National Academy of Sciences, 1982
- Endonuclease recognition sites mapped on Zea mays chloroplast DNAProceedings of the National Academy of Sciences, 1976
- Duplicate genes for tyrosine transfer RNA in Escherichia coliJournal of Molecular Biology, 1970