ALLOSPECIFIC MONOCLONAL ANTIBODIES RECOGNIZING RAT CLASS I AND CLASS II HISTOCOMPATIBILITY ANTIGENS

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Abstract
Rat-mouse hybridomas were developed producing monoclonal antibodies which recognize rat major histocompatibility complex specificities. Splenic lymphocytes from a LEW (RT1l) rat alloimmunized against ACI (RTla) splenocytes were used for hybridoma production. The 6 clones presented demonstrate 2 patterns of alloreactivity against ACI tissues: clone 36.3 produces an antibody which agglutinates ACI RBC [erythrocyte] and binds to ACI splenic lymphocytes and RBC. Five clones, 65.6, 79.7.1, 79.7.5, 155.3.1 and 189.3.5, from 4 independent wells produce monoclonal antibodies which bind to ACI splenic lymphocytes but not RBC. Screening of these 6 monoclonal antibodies against a reference panel of RBC and lymphocytes from 15 standard, congenic and recombinant rat strains indicated that the monoclonal antibody 36.3 detects a RTI.Aa product (a rat major histocompatibility complex (MHC) Class I antigen) and that 65.6, 79.7.1, 79.7.5, 155.3.1 and 189.3.5 are monoclonal antibodies which recognize a RT1.Ba private specificity (Class II or Ia antigens). These reagents are unique as they are rat monoclonal alloantibodies recognizing private Ia specificities of the rat MHC.