THE SIMULTANEOUS MONITORING OF PLASMA-LEVELS OF NEOSTIGMINE AND PYRIDOSTIGMINE IN MAN

  • 1 January 1980
    • journal article
    • research article
    • Vol. 2  (2) , 77-82
Abstract
The anticholinesterase (Anti-ChE) agents, neostigmine and pyridostigmine, are the most widely used compounds in the treatment of myasthenia gravis. The synthesis of a series of pyridostigmine analogs was reported. From the analogs N,N-dipropylcarbamoyloxy-1-methylpyridinium bromide was considered the most suitable compound for use as a common internal marker for the simultaneous determination of neostigmine and pyridostigmine in human plasma. The assay involved a preliminary ion-pair extraction of the drugs and the internal marker from plasma using K-L glycine buffer. The extract was analysed by a gas chromatography system (10% OV-17 on chromosorb W-AW, 100-120 mesh) linked to an N-sensitive detector. The calibration graphs of neostigmine and pyridostigmine were linear and reproducible over the range 5-100 ng/ml in 3 ml plasma samples. This assay procedure was used to monitor simultaneously the plasma levels of neostigmine (4.7-33 ng/ml) and pyridostigmine (2.7-18.6 ng/ml) of a myasthenic patient over a period of 12 h with repeated dosing of neostigmine bromide (30 mg) and pyridostigmine bromide (60 mg).

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