A thermostable, alkaline-active, keratinolytic proteinase from chrysosporium keratinophilum
- 1 September 1994
- journal article
- Published by Springer Nature in World Journal of Microbiology and Biotechnology
- Vol. 10 (5) , 563-567
- https://doi.org/10.1007/bf00367668
Abstract
Thermostable alkaline proteinase was produced by a strain of Chrysosporium keratinophilum when cultured in lactose/mineral salt medium incorporating keratin solubilized with DMSO. The proteinase, partially purified by cold-acetone precipitation followed by gel-filtration on Sephadex G-75, was optimally active at pH 9 and stable from pH 7 to 10 with over 90% relative residual activity after incubation at 25°C for 24 h. The optimum temperature for enzyme activity was 90°C at which the activity half-life was 30 min. Enzyme activity was stimulated by Fe2+ and inhibited by 1,10 o-phenanthroline. Gel-filtration indicated an M r of 69 kDa.Keywords
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