Pollen-stigma Interaction in the Leguminosae: Constituents of the Stylar Fluid and Stigma Secretion of Trifolium pratense L.

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Abstract
The style of T. pratense is hollow, and the canal contains a watery secretion which forms the medium through which the pollen tubes grow after penetrating the stigma head. In self-incompatible genotypes, incompatible pollen germinates freely and the tubes penetrate the stigma, but they are arrested in the canal after passing an inflated zone (entasis) proximal to the stigma head. The stylar fluid contains sucrose, glucose and traces of galactose and arabinose, as well as a range of proteins. Comparison of the proteins in the stigma eluate and stylar fluid by microgradient gel electrophoresis shows that the spectra are broadly similar; but in addition to various minor differences, two major glycoproteins are present in the stigma secretion which are absent from the style, while one in the stylar fluid is not represented in the stigma. Six esterase isoenzymes are present in the stylar fluid, and three of these also in the stigma eluate; there are also differences in acid phosphatase isoenzymes.

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