Immunocytochemical detection of fungi in the roots of Rhododendron

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Abstract
Antisera were prepared from fruiting structures of a Clavaria sp. found growing in the vicinity of Rhododendron plants and from mycelium of Pezizella ericae grown in culture. The antisera were used for the detection and differentiation of fungi in thin sections of roots of Rhododendron by an indirect immunocytochemical procedure using colloidal gold-labelled goat antirabbit immunoglobin G. Three distinct fungal types could be detected in roots: hyphae that reacted only with the Clavaria antiserum; hyphae that reacted only with the Pezizella antiserum; and hyphae that did not react with either antiserum.

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