Cleavage map of bacteriophage X174 RF DNA by restriction enzymes

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Abstract
ϕX RF DNA was cleaved by restriction enzymes from Haemophilus influenzae Rf (Hinf I) and Haemophilus haemolyticus (Hha I). Twenty one fragments of approximately 25 to 730 base pairs were produced by Hinf I and seventeen fragments of approximately 40 to 1560 base pairs by Hha I. The order of these fragments has been established by digestion of Haemophilus aegyptius (Hae III) and Arthrobacter luteus (Alu I) endonuclease fragments of ϕX RF with Hinf I and Hha I.