Biosynthesis of the polygalacturonic acid chain of pectin by a particulate enzyme preparation from Phaseolus aureus seedlings.

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Abstract
A participate preparation from Phaseolus aureus seedlings which catalyzes the formation of radioactive pectin from UDP-D-galacturonic acid-C14 is described. The biosynthetic polygalacturonate was found to be completely hydrolyzed with Penicillium chrysogenum polygalacturonase to D-galacturonic acid-C14. partial degradation of the radioactive biosynthetic polygalacturonic acid by the action of an exopolygalacturonic acid transeliminase produced radioactive unsaturated digalacturonic acid. Since the pattern of action of this transeliminase is known, the production of this radioactive degradation product implies that the radioactive pectin is formed by successive additions of radioactive D-galacturonic acid residues. Approximately 65% of the radioactive D-galacturonic acid from UDP-D-galacturonic acid-C14 could be incorporated into pectin by this mung bean enzyme system.
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