Automated Chemical Synthesis of a Protein Growth Factor for Hemopoietic Cells, Interleukin-3
- 10 January 1986
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 231 (4734) , 134-139
- https://doi.org/10.1126/science.3079915
Abstract
Interleukin-3 (IL-3), a protein of 140 amino acids, was chemically synthesized by means of an automated peptide synthesizer and was shown to have the biological activities attributed to native IL-3. Assays of synthetic analogues established that an amino terminal fragment has detectable IL-3 activity, but that the stable tertiary structure of the complete molecule was required for full activity. The results demonstrate that automated peptide synthesis can be applied to the study of the structure and function of proteins.Keywords
This publication has 35 references indexed in Scilit:
- Molecular cloning of cDNA for murine interleukin-3Nature, 1984
- Chemical mechanism to account for artifactual formation of shortened peptides with free alpha‐amino groups in solid phase peptide synthesisInternational Journal of Peptide and Protein Research, 1983
- Formation of covalent hybrids from amino terminal and carboxy terminal fragments of two ovomucoid third domainsBiochemistry, 1983
- Molecules stimulating early red cell, granulocyte, macrophage, and megakaryocyte precursors in culture: Similarity in size, hydrophobicity, and chargeJournal of Cellular Physiology, 1982
- Synthesis of N‐tert.‐butoxycarbonyl(α‐phenyl)aminomethylphenoxyacetic acid for use as a handle in solid‐phase synthesis of peptide α‐carboxamidesInternational Journal of Peptide and Protein Research, 1981
- A new synthetic route to tert-butyloxycarbonylaminoacyl-4-(oxymethyl)phenylacetamidomethyl-resin, an improved support for solid-phase peptide synthesisThe Journal of Organic Chemistry, 1978
- Totalsynthese von Humaninsulin unter gezielter Bildung der Disulfidbindungen. Vorläufige MitteilungHelvetica Chimica Acta, 1974
- Rapid solid-phase synthesis of bradykininBiochemical and Biophysical Research Communications, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963