Estimation of urinary 5‐hydroxytryptamine‐O‐glucuronide, a metabolite of endogenous 5‐hydroxytryptamine in sheep

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Abstract
1 5-Hydroxytryptamine-O-glucuronide has been extracted from sheep urine and characterized by its chemical reactions and RF values on paper chromatograms. 2 5-Hydroxytryptamine-O-glucuronide in urine was separated from an inhibitor of β-glucuronidase using an anion exchange resin, and then estimated by measurement of the 5-hydroxytryptamine (5-HT) liberated on incubation with β-glucuronidase. 3 About 20% of the 5-hydroxyindoles found in sheep urine was 5-HT conjugated with glucuronic acid (1.18 ± 0.08 mg 5-HT/24 h, thirty experiments). 4 Sheep urine also contained free 5-HT (0.35 ± 0.04 mg/24 h, thirty experiments) and 5-hydroxyindolylacetic acid (4.43 ± 0.33 mg/24 h, thirty experiments). 5 5-Hydroxytryptamine-O-sulphate was not found in sheep urine.