Evidence of the existence of a high molecular weight form of DNA polymerase .ALPHA. in sea urchin eggs.
- 31 December 1985
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 11 (3) , 311-316
- https://doi.org/10.1247/csf.11.311
Abstract
DNA polymerase .alpha. combined with the endoplasmic reticulum (ER) was isolated from unfertilized sea urchin eggs. NaCl treatment of this fraction released DNA polymerase .alpha. from the ER. The molecular size (the S value) of the ER-free DNA polymerase .alpha. changed with the concentration of NaCl used; being 23 S, 11-15S 11-15S and 6-8S in the presence of 0.05-0.12 M, 0.12-0.24 M and more than 0.24 M NaCl. DNA polymerase .alpha. activity decreased concomitantly with the reduction in molecular size. The 6-8 S form of DNA polymerase .alpha. did not aggregate by itself nor with other cellular components nonspecifically, when the 23 S form was present. These results are evidence of the presence of 6-8 S DNA polymerase .alpha. as a high molecular weight form (23 S-form) in sea urchin eggs.This publication has 0 references indexed in Scilit: